杧果炭疽病抗性杂交群体的构建及SSR分子标记鉴定

Construction of the resistance to anthracnose for crossed mango population and identification by SSR molecular markers

以对炭疽病高抗的金煌品种为母本,高感的爱文品种为父本构建杧果炭疽病抗性杂交群体,共获得杂交果实219个,培育杂交实生种苗184株。按花朵统计结实率为2.18%。通过对184株杂交种苗进行SSR分子标记鉴定,结果表明,16对引物中15对能够有效扩增产物;12对具有丰富的多态性;8对在父母本之间具有多态性,184株杂交种苗可以确认是真实杂种,应用SSR分子标记技术可以对杧果杂种的真实性进行快速验证。

An anthracnose resistant crossed population was established with highly resistant cultivar Chun Hwang as maternal plant and highly susceptible cultivar Irwin as paternal plant,resulted in 219 crossed fruit and 184 crossed seedlings.And the fruit set rate was 2.18%,calculated by flowers.Among the 16 primers used,15 primers could amplify effective band;12 primers gave stable profiles and 8 primes showed higher polymorphism information content between the parent plants.In ad-dition,184 true hybrids were selected by SSR molecular markers.The results showed that SSR molecular markers were a very sensitive and effective tool for mango hybrids verification.