原子转移自由基聚合法改性壳聚糖及其抑菌性研究

Preparation of novel chitosan by the atom transfer radical polymerization and study on the antibacterial activity

合成了大分子引发剂(CS-Br)———壳聚糖(CS)接枝2-溴丙酰溴,然后以溴化亚铜与五甲基二乙烯三胺(PMDETA)为催化体系,氯甲基化甲基丙烯酸二甲氨乙酯季铵盐(DMC)为单体,利用原子转移自由基聚合法制备了新材料P(CS-Br-DMC)。重点研究了该材料对白色念珠菌、金黄色葡萄球菌和大肠杆菌的最低抑菌浓度(MIC)、抑菌圈直径及溶血性。同时考察了其在不同时间对三种菌种生长的影响,并且与壳聚糖本身的抑菌性进行了对比。结果表明,该材料比壳聚糖具有更好的水溶性和抑菌性,其对金黄色葡萄球菌的抑菌效果最佳。

The macroinitiator （CS-Br） was composed with chitosan （CS） and 2-bromopropionyl bromide. Then, with CS-Br as macroinitiator and CuBr/ N, N, N＇, N＇, N＂ -pentamethyldiethylenetriamine （PMDETA） as a catalyst, a novel material P （ CS-Br- DMC）was successfully prepared with CS-Br and chlorine methylation dimethyl ammonium ethyl methacrylate（DMC） via atom transfer radical polymerization （ATRP）. The minimal inhibitory concentration （ MIC）, inhibition zone and hemolysis of Candida albicans, Staphylococcus aureus and Escherichia coli were focused on. Meanwhile,the antibacterial activity of the materials through the different time on the growth of three colonies were investigated. Furthermore, its water solubility and MIC was compared with CS. The findings indicated that the water solubility and antibacterial activity of new substance was better than that of CS especially for Staphylococcus aureus.