偶联剂N-β-(氨乙基)-γ-氨丙基三甲氧基硅烷对多孔陶瓷的表面改性

Surface modification of ceramics with the coupling agent N-β-aminoethyl-γ-aminopropyltrimethoxysilane

用偶联剂N-β-(氨乙基)-γ-氨丙基三甲氧基硅烷(AEAPTS)对网状多孔陶瓷表面改性,改性陶瓷用于辣根过氧化物酶的固定化。采用X射线能谱法对改性后的陶瓷表面元素进行了分析,结果表明AEAPTS向陶瓷表面引入了氨基;对陶瓷表面改性的研究结果表明,最适工艺条件为活化陶瓷的盐酸浓度2.5mol/L,改性剂AEAPTS质量分数15.0%,温度70℃,pH为4.5,反应时间3.5 h。在此条件下,1 g陶瓷与改性剂的最大结合量为2.13 g。

The surface of reticulated porous ceramics to be used for immobilization of enzymes was modified by the coupling agent N-β-aminoethyl-γ-aminopropyhrimethoxysilane （AEAPTS）. Energy-dispersive X-ray spectrometry （EDS） showed that AEAPTS had been successfully grafted on the surface of the ceramics. The surface of the ceramies was bound to the amino-group of AEAPTS. The results indicated that the optimum conditions for grafting were as follows： concentration of hydrochloric acid used for activation of 2.5 mol/L, mass fraction of AEAPTS of 15.0% , pH of 4.5, temperature of 70 ℃, and reaction time of 3.5 h. Under these conditions, 1 g of ceramics could be linked to the maximum amount of of coupling agent, namely 2. 13 g.