冬凌草甲素对HL-60细胞p53表达及细胞周期的影响

Effects of rebescensine A on HL-60 cell expression of p53 and cell cycle changed

目的探讨冬凌草甲素对HL-60细胞p53表达及细胞周期的影响。方法通过PI染色,流式细胞仪检测细胞周期和凋亡峰,并以RT-PCR检测p53基因的表达。结果流式细胞仪分析显示,冬凌草甲素处理后使细胞周期发生变化:G1期、S期细胞的比例减少,G2/M期细胞的比例增加,凋亡的细胞数比例增多,且随着药物浓度的增加而逐渐增加。RT-PCR检测结果显示,20μmol/L冬凌草甲素作用后,与对照组相比,HL-60细胞p53 mRNA水平显著升高,且随时间延长表现得更为明显。结论冬凌草甲素能使细胞周期发生变化和p53基因的上调,从而诱导细胞发生凋亡。

Objective To investigate the effects of rebescensine A on HL-60 cell expression of p53 and cell cycle.Methods HL-60 cells in culture medium in vitro were given different concentrations of rebescensine A.Propidium I dide（PI） binding and flow cytometry were used to detect cell cycle and apoptotic peak;And RT-PCR was used to observe the expression of p53.Results Marked morphological changes of cell apoptosis were observed.Cell cycle changed after the cells treated by rebescensine A： The ratio of G1 and S-phase cells decreased,G2 and M-phase cells increased and apoptotic cells count increased along with the increasing concentrations of rebescensine A.The up-regulated of p53 mRNA were showed by RT-PCR and changed more obviously with prolongation in HL-60 cells treated by rebescensine A 20 μmol/L.Conclusion Rebescensine A could change the cell cycle of HL-60 cells,and the up-regulated of p53 gene,which induced apoptosis.