熟地泽泻药对配比改变对庆大霉素耳毒性离体细胞模型的作用比较

Otoprotectant Activities Comparison of Shudi and Zexie in Different dose proportion on Gentamycin Induced Ototoxic Organotypic Cultures

目的:观察熟地(radix rehmanniae preparata)与泽泻(Rhizoma Alismatis)组成的药对在不同剂量配比下对庆大霉素(GM)损伤毛细胞的不同保护作用,寻找其最佳剂量配比。方法:复制内耳螺旋器体外培养GM损伤模型,参照基线等比增减设计,对熟地、泽泻设8个剂量配比组,通过耳蜗毛细胞计数观察不同剂量配比下熟地、泽泻对GM损伤耳蜗内、外毛细胞损失率的影响。结果:全耳蜗计数结果显示,熟地泽泻在8∶3、8∶8、0∶3、0∶8配比下,外毛细胞损失率在全耳蜗范围内较GM模型组有明显下降(P<0.01)。其他配比组与模型组无统计学差异。结论:熟地泽泻在不同配比下对毛细胞损失率的影响呈现出一种非线性的量效关系,原方配比(8∶3)及其他配比(8∶8、0∶3、0∶8)属最佳配比。

Objective：To investigate the protective effects of Shudi （radix rehmanniae preparata）-Zexie （Rhizoma Alismatis） prescriptions in different proportion on gentamycin damaged hearing hair cells. Methods：The model of gentamicin induced ototoxicity in cochlear cultures was copied. Cochlear organotypic cultures of postnatal day 3~5 （P3~P5） mice were treated with gentamycin alone or in combination with eight respective Shudi-Zexie prescriptions in different proportion. The effects of the prescriptions on gentamicin induced hair cell loss were observed. Result：Hair cell counting data of the whole organ of corti showed that,compared with the model group,the combination of Shudi and Zeixe in ratio of 8∶3,8∶8,0∶3 and 0∶8 can decrease the hair cell loss ratio significantly （P0.01）,while there showed no obvious difference of the other descriptions.Conclusion：The general influence on the gross cell damage rate of the combination of Shudi and Zexie showed a nonlinear dose-effect relationship. The original recipe （8∶3） and the other prescriptions （8∶8、0∶3、0∶8） beongs to the most optimal proportion scales.