HPLC法同时测定参麦地黄丸中丹皮酚芍药苷和马钱苷的含量

Determination of Paeonol Paeoniflorin and Loganin in Shenmaidihuang Pills by HPLC

目的:建立一种HPLC方法同时测定参麦地黄丸中丹皮酚、芍药苷和马钱苷的含量。方法:采用HPLC法,色谱柱为Agilent Zorbax SB-C18(4.6mm×250mm,5μm)柱,流动相:以乙腈-水为流动相,进行梯度洗脱;流速:1.0mL.min-1;柱温:30℃;检测波长:236nm(0～8min)→230nm(8～12min)→274nm(12～30min)。结果:丹皮酚、芍药苷和马钱苷线性范围分别为8.17～163.36μg·mL-1(r=0.9999),2.37～48.54μg·mL-1(r=0.9997)和2.71～31.82μg·mL-1(r=0.9998),平均加样回收率分别为98.62%(RSD=1.78%),97.58%(RSD=0.91%)和99.00%(RSD=2.56%)。结论:新建方法简便、准确、易行,可用于控制制剂质量。

Objective：To establish a HPLC method for the simultaneous determination of paeonol,paeoniflorin and loganin in Shenmaidihuang pills. Methods：A Agilent Zorbax SB-C18（4.6 mm×250mm,5μm）column was used with the mobile phase of acetonitrile-water at a flow rate of 1.0 mL·min-1 and a column temperature of 30℃. The eluted analytes were detected by on-line UV detector whose wavelength was set at 236 nm from 0 to 8 min,230 nm from 8 to 12 min and 274 nm from 12 to 30min. Results：The calibration curves of paeonol ,paeoniflorin and loganin were linear over the ranges of 8.17～163.36μg·mL-1（r=0.9999）,2.37～48.54μg·mL-1 （r=0.9997） and 2.71～31.82μg·mL-1 （r=0.9998）,respectively,and the average recoveries were 98.62%（RSD=1.78%）,97.58%（RSD=0.91%） and 99.00%（RSD=2.56%）,respectively.Conclusions：This method was simple,accurate,feasible and can be can be used for drug control.