路路通HPCE指纹图谱的建立及其质量研究

Establishment of Chromatographic Fingerprint and Quality Assessment of Fructus Liquidambris by HPCE

目的:建立路路通的高效毛细管电泳指纹图谱,研究不同产地路路通药材的质量,建立评价路路通质量优劣的指纹图谱分析方法。方法:利用HPCE方法,采用未涂层熔融石英毛细管柱(57cm×75μmID,有效长度50cm)为分离通道,以12mmol·L-1硼砂:甲醇(80:20)为电泳介质,分离电压30kV,毛细管温度30℃,检测波长200nm。结果:确定了路路通药材中的10个共有峰,初步体现路路通药材的特征性。结论:路路通药材的高效毛细管电泳指纹图谱,重复性好,专属性强,用于路路通的质量评价切实可行,可结合含量测定用于全面控制路路通药材的质量。

Objective：To establish the chromatographic fingerprint analysis for the quality control of Fructus Liquidambris. Methods：HPCE method was applied to establish the chromatographic fingerprint. An uncoated fused silica capillary （57cm×75μm ID,effective length of 50cm） was used. The electrolyte buffer composed of 12mmol·L-1 borax-methanol （80：20）,applied voltage 30kV,the capillary temperature was 30℃,detection wavelength was 200nm. Results：The HPCE chromatographic fingerprint of Fructus Liquidambris showing 10 common peaks was established.,and showing ten common peaks represented the characteristics of the herb＇s constituents. Conclusions：The HPCE fingerprint method is repeatable,feasible in analysis of Fructus Liquidambris and was proved to be a reliable and rapid method for the quality control of Fructus Liquidambris.