摘要
目的观察灰树花多糖对小鼠免疫功能和辐射损伤的影响。方法将小鼠随机分为溶剂对照组和3个试验剂量组,剂量分别为0.15g/kg、0.30g/kg、0.90g/kg,每天灌胃給予,连续30d后,检测7项免疫指标。另取小鼠,按体重随机分为辐射对照组和3个试验剂量组,剂量设计和给药方式同上,30d后,各组均以^(60)Co-γ射线进行一次全身照射,根据检测指标选择不同的照射剂量。辐照前、辐照后第3和14天进行外周血白细胞计数;辐照后第3天进行骨髓细胞DNA含量检测:辐照后第7天进行血清超氧化物歧化酶(SOD)活力测定。结果灰树花多糖能显著提高小鼠的抗体生成细胞数和血清溶血素水平,ConA诱导的脾淋巴细胞增殖能力和NK细胞活性明显增强,碳廓清指数、腹腔巨噬细胞吞噬指数明显高于对照组。灰树花多糖能显著提高受^(60)Co-γ射线照射小鼠的外周血白细胞数和骨髓细胞DNA含量,明显增强血清超氧化物歧化酶(SOD)活力。结论灰树花多糖能增强小鼠的细胞免疫和体液免疫功能以及单核-巨噬细胞吞噬功能,对辐射损伤具有一定的防护作用。
Objective To investigate the influences for Maitake Polysaceharide on the Immunoregulation and anti - radiating damage. Methods The mice were divided into control group and three experiment groups. The mice of three experiment groups were fed Maitake Polysaccharide at dose of 0.15g/kg,0.30g/kg,0.90g/kg for 30 days. 7 indexes were observed to detect the effect of Maitake Polysaeeharide on the immunoregulation. The other mice were divided into radiating control group and three experiment groups. The mice of three experiment groups were fed in the same way. Then all animals were radiated by ^60Co-γ. The number of white blood ceils（WBC） was counted before radiating , on 3 and 14 day after radiating. The DNA content of bone marrow cells were measured on 3 days after radiating. The activities of superoxide dismutase（SOD） in serum were measured on 7 days after radiating. Results Maitake Polysaccharide could increase the amount of hemolytic antibody in serum. The proliferation of ConA - induced mitogenic response for spleen lymphocyte was also increased. Maitake Polysaccharide could enhance the activity of NK cell and the ability of phagocytosis significantly. Compared with the radiating control group, the number of WBC and the DNA content of bone marrow ceils, and the activities of SOD in serum increased significantly. Conclusion Maitake Polysaccharide can improve cell - mediated and humoral immune function and non - specific immune function, and protect the mice from radiation injuries.
出处
《中国辐射卫生》
2010年第1期6-8,共3页
Chinese Journal of Radiological Health
