热量限制对老龄大鼠血清SOD及MDA的影响

Effect of caloric restriction on SOD and MDA in serum of aged rats

目的研究热量限制对老龄大鼠血清SOD及MDA的影响,为完善人类健康的饮食方式提供实验基础。方法45只24月龄雌性健康SD大鼠随机分成3组:对照组(正常饮食组)、1∶1组(隔日喂养组)、5∶2组(进食5d,禁食2d组)。不同饮食方式干预3月后采集标本,测定大鼠血清SOD活性和MDA含量。结果与对照组相比较,1∶1组和5∶2组SOD活性显著增高(P分别<0.01,<0.05),MDA含量均显著降低(P<0.01)。1∶1组与5∶2组相比较,SOD活性及MDA含量差异无统计学意义(P>0.05)。结论对老年大鼠进行热量限制,能明显提高SOD活性,降低MDA含量,改善氧化应激水平。

Objective To explore the effect of caloric restriction on the superoxide dismutase （SOD）activity and malondialdehyde （MDA） content in the serum of aged rats,and to provide a experimental basis for improving the healthy diet of the human. Methods Forty-five female 24-month old SD rats were randomly divided into 3 groups ： control group（ normal feeding）, 1 ： 1 group（ feeding for 1 d, fasting for 1 d） and 5： 2 group （ feeding for 5 d, fasting for 2 d）. Then the serum levels of SOD and MDA were measured with reagent bo- xes after dietary intervention for 3 months. Results Activity of SOD was significantly higher in l： 1 group（P 〈 0.01 ） and 5：2 group （P 〈 0.05 ）than that in control group, while the contents of MDA were significantly lower （P 〈 O. 01 ）. The SOD activity and the con- tents of MDA showed no sighificant difference between 1 ： 1 group and 5：2 group （P 〉 O. 05）. Conclusion Caloric restriction can in- crease the activity of SOD and decrease the content of MDA in the aged rats,resulting in the attenuation of oxidative stress.