富含共轭亚油酸的食用油安全性毒理学试验研究

Conjugated linoleic acid-rich oil of safety research toxicological tests

目的日本已经有富含共轭亚油酸的食用油商品。为了使共轭亚油酸在我国得到广泛的应用,进行富含纯度共轭亚油酸食用油的食品安全性毒理试验研究。方法依据GB15193-94《食品安全性毒理学评价程序和方法》进行急性毒性试验、三项遗传毒性试验和30天喂养试验。结果共轭亚油酸对两种性别的SPF级昆明种小鼠经口毒性,一次灌胃量达20g/kg.bw两周内动物未见明显中毒症状,无动物死亡,按急性毒性分级标准规定,该受试物属无毒级。三项遗传毒性试验(Ames、小鼠骨髓嗜多染红细胞微核试验、小鼠精子畸形试验)结果均为阴性。30天喂养试验结果表明:该受试物0.87、2.20、4.30g/kg.bw剂量(分别相对于推荐人群日摄影入量3.6g/kg.bw的20、50、100倍)对wistar大鼠的临床检查,血液学,生化学,脏器重量和系数以及病理组织多样指标无明显影响,未发现该受试物有明显的毒性作用。结论富含共轭亚油酸的食用油既无毒也无任何副作用。

The purpose of Japan has been rich in conjugated linoleic acid in edible oil products. In order to conjugated linoleic acid in China has been widely used for purity conjugated linoleic acid-rich oil of the food safety research toxicological tests. Methods GB15193-94 ＂Toxicological evaluation of food safety procedures and methods＂ for acute toxicity tests, the three genetic toxicity tests and a 30-day feeding study. The results of conjugated linoleic acid on both sexes of mice SPF level oral toxicity, the first two weeks of gavage 20g/kg.bw reached no obvious symptoms of poisoning animals, no animal deaths, accord- ing to the acute toxicity classification standards, the test material is non-toxic level. The three genetic toxicity tests （Ames, mouse bone marrow polychromatic erythrocytes micronucleus test, sperm shape abnormality test in mice） were negative. 30-day feeding study results show that： The subjects were 0.87,2.20,4.30 g / kg.bw dose （respectively, compared with the recommended amount of people into photography 3.6g/kg.bw on the 20,50,100-fold） of wistar rats Clinical examination, hematology, biochemistry, organ weight and histopathological factors as well as a variety of indicators had no significant effect, the subjects were not found significant toxicity. Conclusion of conjugated linoleic acid-rich oil is non-toxic and no side effects.