红树林放线菌的筛选与抗真菌和抗肿瘤活性的测定

The Antifungal and Antitumor Activities of the Isolated Mangrove Forest Actinomycetes

通过从深圳福田红树林根际于不同季节采集的3份土样和2份水样中分离得到95株放线菌,94%的放线菌为链霉菌属。测定95株放线菌发酵液对9种病原真菌的抗菌活性,其中31.6%的放线菌发酵液有不同程度的抗病原真菌作用。同时采用MTT法测定了它们对肺癌细胞A549、白血病细胞株K562两种肿瘤细胞的细胞毒活性,其中21%放线菌发酵液具有细胞毒活性。s33、s62、s65和s68这个4株放线菌发酵液具有抑制5种以上真菌的作用,并且对两种肿瘤细胞株均有作用。

The obective of this study is to isolate mangrove forest derived actinomyces strains for obtaining the bioactive metabolite producing strains to investigate antifungal and anti-tumor active metabolites.For this,actinomyces strains were isolated by single colony isolation technique.The antifungal activity was assayed by paper method.The anti-tumor activity was assayed by MTT method.Ninety-five strains of actinomyces were isolated from 3 soil samples and 2 water samples collected from a mangrove forest in Futian,south of Shenzhen,China,of which 94% were streptomycetaceae.Nine pathogens were used to test the antifungal activity of the ninety-five strains,among of them 31.6% of the fermentation broth showed the antifungal activity.Two tumor cell lines（A549 and K562） were used to test the antitumor activity with the MTT method.The result indicated that 21% of the fermentation broth presented the antitumor activity.Furthermore,the fermentation broth of the s33,s62,s65and s68 showed antifungal activity toward more than five pathogens and antitumor activity on the two cell lines.