基于核壳型荧光纳米颗粒检测的人免疫球蛋白G免疫分析方法研究

Fluoroimmunoassay for Human IgG Using Ru(bpy)_3Cl_2-doped Fluorescent Silica Nanoparticles as Labels

生物分子标记的荧光检测技术已广泛应用于生物学检测和疾病诊断。实验利用反向微乳液技术,制备出Ru（bpy）3Cl2掺杂SiO2荧光纳米粒子。IgG的检测是基于IgG和荧光纳米颗粒标记的羊抗人IgG的特异性结合。实验中,IgG检测的线性范围为1-100 ng/mL,检出限达到0.3ng/mL,对30 ng/mL人IgG进行11次检测,相对标准偏差为2.2%。实验结果表明,该检测新方法具有检测灵敏度高、操作简单和重复性好。

Fluorescent-labeled molecules have been widely used in biological detection and diagnosis.In this manuscritpt,the Ru（bpy）32＋-doped silica nanoparticles were firstly prepared with a modified W/O method.Human IgG（hIgG） was measured based on the specific interaction between hIgG and functionalized fluorescent core-shell nanoparticle-labeled gost anti-hIgG.The calibration graph for hIgG was linear over the range 1-100 ng/mL with a detection limit of 0.3 ng/mL.From 11 measurements,the R.S.D.is 2.2% for samples of 30 ng/ mL of hIgG.The results demonstrated that the method have a potential advantages of sensitivity,simplicity and good reproducibility for the determination of hIgG,and is applicable to the determination of hIgG in serum samples.