摘要
初步研究了摇瓶条件下地衣芽孢杆菌发酵生产3-羟基-2-丁酮的工艺条件。分析了摇瓶发酵中菌体生长、产物合成和pH的变化情况;考察了发酵温度、装液量、种龄、接种量等条件对发酵的影响;研究了地衣芽孢杆菌利用不同碳源发酵生产3-羟基-2-丁酮的情况,选取了最适碳源,在此基础上进行了补糖试验;最后采用正交试验方法对培养基进行了优化。在优化的发酵条件下,采用分批补糖的工艺,摇瓶发酵产量可稳定在20 g/L以上,为3-羟基-2-丁酮的工业化生产提供了一定的基础。
The nutritional and environmental conditions for acetoin production from Bacillus licheniformis in flasks were studied in this manuscript.Firstly,the environmental conditions,such as pH value,culture temperature,seed age,volume and inoculation ratio,and nutritional conditions,like carbon and nitrogen sources were carefully investigated.Based on the results of the single factor experiment,the orthogonal experiment was carried to get the optimum medium composition.Then fed-batch fermentation was performed with glucose feeding.The yield of acetoin achieved at 20 g/L in fed-batch fermentation with glucose feeding.
出处
《食品与生物技术学报》
CAS
CSCD
北大核心
2010年第1期155-160,共6页
Journal of Food Science and Biotechnology
