吗啡对大鼠脑缺血再灌注后脑含水量及S100β的影响

Effects of morphine on brain water content and S100beta in rats with cerebral ischemia-reperfusion injury

目的:探讨吗啡预处理对大鼠脑缺血再灌注损伤后脑含水量及脑脊液S100β的影响。方法:Pusinelli方法建立四动脉阻断全脑缺血模型:成年雄性大鼠36只,随机分为假手术组(n=12)、脑缺血再灌注组(n=12)、吗啡预处理组(n=12)。脑缺血8min,再灌注48h后,每组取6只大鼠断头取脑制作石蜡切片,HE染色观察海马区组织病理学改变;另取剩余6只大鼠于枕骨大孔抽脑脊液后立即断头取脑,干湿称重法测量脑含水量,酶联免疫吸附法检测脑脊液中S100β蛋白含量。结果:吗啡预处理组细胞受损的病理学改变显著轻于脑缺血再灌注组。吗啡预处理组脑含水量及S100β蛋白含量明显低于脑缺血再灌注组(P<0.05或P<0.01)。结论:吗啡预处理可减轻缺血性脑损伤,提高脑缺血耐受性。

Objective：To investigate effects of morphine preconditioning on brain water content and S100beta of cerebrospinal fluid（CSF） in rats with cerebral ischemia-reperfusion injury.Methods：36 male rats were randomed divided into three groups：sham-operated group（n=12）;cerebral ischemia-reperfusion group（n=12）;morphine preconditioning group（n=12）.Global cerebral ischemia model were produced by four-vessel occlusion as described by Pusinelli.Ischemia models were made to occlude carotid artery for 8 min,then which were loosened to reperfusion.At the end of 48 h reperfusion,Hippocampus tissue were obtained for HE staining to detect pathological changes and brain were obtained to weigh and CSF were obtained to detect S100beta protein.Results：Pathological changes of neuron in morphine preconditioning group were significantly lighter than that in cerebral ischemia-reperfusion group.Compared with that in cerebral ischemia-reperfusion group,brain water content and values of S100beta in morphine preconditioning group decreased significantly（P〈0.05 or P 〈0.01）.Conclusion：Morphine preconditioning can reduce ischemic brain injury and improve cerebral ischemic tolerance.