蛋白质磷酸化酶抑制剂对肺癌细胞株的增殖抑制作用

Inhibitory effect of protein phospholipid kinase inhibitor on porliferation of lung cancer cell lines

目的探讨蛋白质磷酸化酶抑制剂对肺癌细胞株的增殖抑制作用。方法用蛋白质磷酸化酶抑制剂对６株小细胞肺癌和７株非小细胞肺癌细胞株的增殖抑制效果进行了检测，并采用免疫法，测定了蛋白质磷酸化酶抑制剂对肺癌细胞内Ｒｂ蛋白表达的影响。结果随其浓度的增加，对肺癌细胞株的增殖抑制作用增强，小细胞肺癌的半数抑制量平均值ＳＴ为９５ｎＭ，ＵＣＮ－０１为５８８ｎＭ，非小细胞肺癌则分别为１８８ｎＭ和１０２９ｎＭ，ＳＴ和ＵＣＮ－０１可抑制肺癌细胞Ｒｂ蛋白的磷酸化。结论蛋白质磷酸化酶抑制剂通过抑制Ｒｂ蛋白的磷酸化。

Objective To study inhibitory effcet of protein phospholipid kinase inhibitor on proliferation of lung cancer cell lines.Methods The inhibitory effect of the proliferation were investigated in 6 small cell lung cancer cell lines and 7 non small cell lung cancer cell lines by Use of phospholipid kinase inhibitor Staurosporine and UCN 01.And the expression of Rb protein were detected in lung cancer cell lines with Westein blotting method.Results Staurosporine and UCN 01 stuongly inhibited the growth of lung cancer cell lnes.The median inhibitor dose of Staurosporine and UCN 01 stuongly inhibited the growth of lung cancer cell lines.The median inhibitor dose of Staurosporine and UCN 01 in small lung cancer cell lines was 95nM,588nM,respectively,in non small lung cancer cell lines,was 188nM,1029nM,respectively,The expression of Rb protein were strongly inhibited by Staurosprine and UCN 01.Conclusion The inhibitory effect of Staurosporine and UCN 01 on proliferation of lung cancer cell lines were proceeded by the inhibition of phospholipid Rb protein.