建立夹心ELISA法测定纤维蛋白(原)降解产物E碎片的初步临床应用

Determination of fibrin(ogen) degradation fragment E and its preliminary clinical application

目的为探索纤维蛋白（原）Ｅ碎片（ＦＤＰ－Ｅ）测定的临床意义。方法通过自制ＦＤＰ－Ｅ单克隆抗体建立夹心ＥＬＩＳＡ测定ＦＤＰ－Ｅ碎片的含量，同时结合Ｄ－Ｄｉｍｅｒ指标，测定２２例成人组，１５例小儿组以及４２例肝病组，４７例小儿肾脏疾病组患者。结果肝病和小儿肾脏疾病患者ＦＤＰ－Ｅ、Ｄ－Ｄｉｍｅｒ均明显升高，两者呈显著正相关（ｒ＝０．７８３６、０．６９１７）。

Objective In order to estimate the clinical value of determination of fibrino(gen)degradation products E.Methods The amounts of FDP E were determined by using sandwich ELISA assay which was established by self made McAb.In combination with the determination of D Dimer,we investgated the both tests in 22 normal adults,15 normal children and 42 patients with liver disease,47 child ren with nephritis.Resuts The significantly elevated levels of both FDP E and D Dimer were presented in liver diseaseand child nephritis,and the correlation between FDP E and D Dimer was highly significant(r=0 7836,0 6917).Conclusion It has been suggested that FDP E is also likly to be the molecular marker of fibrinolysis system in thrombotic disease and in hypercoagulabe state of certain involved condition.