雌激素受体β在女性慢传输性便秘患者乙状结肠壁内的分布及其蛋白表达

Distribution and Expression of Estrogen Receptor β Protein in Sigmoid Colon of Female Patients with Slow Transit Constipation

目的研究女性慢传输性便秘(slow transit constipation,STC)患者雌激素受体β(estrogen receptor β,ERβ)在乙状结肠组织的分布及其蛋白表达。方法采用免疫组织化学及Western blot方法检测20例STC患者以及20例对照组患者乙状结肠ERβ的分布及其蛋白表达。结果免疫组化结果显示ERβ在STC组及对照组乙状结肠黏膜、肌间神经丛及黏膜下神经丛均有表达,肌层未见表达。与对照组相比,STC组ERβ表达均明显降低,差异有统计学意义(P<0.05)。Western blot结果显示STC组乙状结肠ERβ蛋白表达较对照组明显降低,差异有统计学意义(P<0.01)。结论肌间及黏膜下神经丛ERβ蛋白表达降低可能参与STC发病。

Objective To investigate the express of ERβ protein in female slow transit constipation （STC） patients. Methods Immunohistochemistry and Western blot technique were used to detect the distribution and expression of estrogen receptor β （ERβ） protein of 20 patients with STC and 20 aged-matched controls. Results ERβ expressions were detected in mucous layer,myenteric nerve plexus and submucous nerve plexus in two groups. In comparison with the control group,the expression of ERβ protein of STC group was much lower （P0.01）. The expression of ERβ protein of sigmoid colon in STC group was significantly lower than that in control group （P0.05）. Conclusion The expression of ERβ protein decreased in myenteric and submucous nerve plexus of sigmoid colon tissues may involve in the pathogenesis of STC.