多种MAGE基因在结直肠癌中的表达和意义

Expression of multiple MAGE genes in colorectal carcinoma

目的探讨多种MAGE基因在结直肠癌组织中的表达及意义。方法通过逆转录聚合酶链反应（1iT—PCR）检测MAGE-A1，MAGE—A3，MAGE—A4和MAGE-A10基因在56例结直肠癌配对癌组织、癌旁组织及四种（SW620，HCT116，HT-29，SW480）结肠癌肿瘤细胞系中的mRNA表达。统计学方法利用SPSS13．0统计软件，采用χ2检验分析。结果MAGE—A1，MAGE—A3，MAGE—A4和MAGE—A10基因在结直肠癌癌组织中的表达分别为5％（3／56），52％（29／56），63％（35／56）和34％（19／56），在癌旁组织中不表达。结肠癌细胞系SW620，HCT116和HT-29分别表达3种MAGE基因，结肠癌细胞系SW480表达2种MAGE基因。89％（50／56）和50％（28／56）的结直肠癌组织分别表达至少一种和两种MAGE基因。MAGE—A4和MAGE—A10基因在TNMⅢ＋Ⅳ期结直肠癌组织中的表达明显高于Ⅰ＋Ⅱ期（P=0．02和P=0．042，P值均〈0．05）。结论MAGE—A3，MAGE—A4和MAGE—A10基因有可能成为结直肠癌免疫治疗的候选靶点；MAGE—A4和MAGE—A10基因可能是结直肠癌的预后标志物。

Objective To explore the expression and significance of multiple MAGE genes in colorectal carcinoma （CRC） patients. Methods The expression of MAGE-A1, MAGE-A3, MAGE-A4 and MAGE-A10 genes were studied by using the reverse transcription polymerase chain reaction （RT-PCR） in 56 CRC patients. Results MAGE-A1, MAGE-A3, MAGE-A4 and MAGE-A10 were expressed at mRNA level in5% （3/56）, 52% （29/56）, 63% （35/56）and34% （19/56）respectively. Colon cancer cell line SW620, HCT116, and HT-29 expressed three MAGE genes respectively and SW480 expressed two MAGE genes. In addition, 89% （50/56） and 50% （28/56） of the 56 samples expressed at least one and two MAGE genes respectively. MAGE-A4 and MAGE-A10 genes expressions are significantly higher in stage TNM Ⅲ ＋ Ⅳ than in stage TNM Ⅰ＋ Ⅱ（P 〈 0. 05）. Conclusions MAGE-A3, MAGE-A4 and MAGE- A10 genes are expressed at a ＇high percentage in CRC, and they are the possible potential targets of immunotherapy. MAGE-A4 and MAGE-A10 genes expressions are significantly correlated with clinical TNM stage.