榄香烯对胃癌细胞p38丝裂原活化蛋白激酶信号通路的影响

Effects of elemene on p38MAPK signal pathway in gastric cancer SGC-7901 cells

目的探讨榄香烯对人胃癌SGC-7901细胞株增殖的影响，以及与p38丝裂原活化蛋白激酶（p38MAPK）信号通路的关系。方法用不同浓度的榄香烯处理胃癌SGC-7901细胞，MTT法检测细胞增殖情况；光镜和透射电镜观察形态学改变；Westernblot方法检测P38和磷酸化P38（p-P38）的表达。结果榄香烯抑制胃癌细胞SGC-7901增殖，并呈浓度和时间依赖性；在光镜和透射电镜下可以观察到典型的细胞凋亡形态学改变；经0．08mg／ml榄香烯作用4h后，胃癌SGC-7901细胞中p38MAPK通路被激活，p-P38表达与对照组相比升高3．72倍，差异有统计学意义（P〈0．01），而P38总蛋白量未发生明显变化。预先应用p38MAPK通路抑制剂SB203580作用24h与单用榄香烯作用组比较，p-P38表达下降54．12％，差异有统计学意义（P〈0．01）。结论榄香烯可以抑制胃癌细胞SGC-7901的增殖，诱发细胞凋亡，其机制可能与p38MAPK通路有关。

Objective To explore the effect of elemene on the proliferation of human gastric cancer cell line SGC-7901 and p38MAPK signal pathway. Methods SGC-7901 cells were treated with various concentrations of elemene. MTTassay was used to detect the proliferation. The morphological changes were observed under light microscope and transmission electron microscope. The expression of P38 and phosphorylated P38 （ p-P38 ） was detected by Western-blot. Results Elemene exerts a potent inhibitory effect on the growth of gastric cancer cells SGC-7901 in a dose-dependent and time-dependent manner. The representative morphological changes of apoptosis were seen under light microscope and transmission electron microscope, p-P38 increased by 3.72-folds following treatment of SGC-7901 cells with 0. 08 mg,/ml elemene for 4 hours, compared with controls, the differences were significant（ P 〈 0. 01 ）. At the same time, the total P38 abundance did not change. Pretreatment of SGC-7901 cells with SB203580, the inhibitor of p38MAPK signal pathway, lowered the level of p-P38 by 54. 12% , compared with controls, the differences were significant（P 〈0. 01 ）. Conclusions Elemene inhibits the proliferation of gastric cancer cells SGC-7901 and induces apoptosis possibly by activating p38MAPK signal pathway.