实验性小白鼠肝脏马尔尼菲青霉菌感染的形态学研究

Morphological Study on Experimental Hepatic Penicillium Marneffei Infection in Mice

将活的马尔尼菲青霉菌的分生孢子５×１０６个从尾静脉接种到Ａ组（ＢＡＬＢ／Ｃ）和Ｂ组（ＢＡＬＢ／Ｃｎｕ／ｎｕＳＩＣ）小白鼠体内，然后按时间依次处死，取其肝脏，用病理组织学及电镜的方法观察其形态学改变。结果表明，在感染早期，Ａ组和Ｂ组变化十分相似，均表现为枯否细胞的吞噬作用及肉芽肿的形成。但随着感染的进一步发展，Ａ组和Ｂ组变化十分悬殊。Ａ组肉芽肿逐渐减少，几乎消失。Ｂ组肉芽肿继续增多，几乎占据整个肝脏。这是因为吞噬孢子的巨噬细胞能否真正杀死真菌，还要依赖于小白鼠的免疫状态，特别是细胞免疫。

Experimental Penicillium marneffei infection in liver of mice was investigated by histopathology and electron microscope.Viable conidia (5×10 6 cells) of P.marneffei were inoculated into each mouse of group A (BALB/c) and group B(BALB/c nu/nu SIC) through the tail vein.All of mice were killed on schedule after inoculation and the livers were examined morphologically.At an early stage of infection,similar pathological changes were observed in group A and B.However,as the infection progressed,there were different changes in them.Whether or not macrophages killed the fungus still depended on the immune function,especially cellular immunity.