摘要
Regeneration from cotyledonary nodes and embryonic tips of soybean ＂Peking＂ was studied. The disinfectant ways of the mercuric chloride and chlorine gas were used and the concentrations of 6-BA and 2, 4-D were studied in the culture medium The results showed that the sterilization effect of chlorine gas was better than that of mercuric chloride. The best concentration of 6-BA was 1.0 mg·L^-1 and the best concentration of 2, 4-D was 2.0 mg·L^-1 in the germinating medium. The number of buds of each explant was 3.56 and 2.98, respectively. The best concentrations of 6-BA and 2, 4-D were 3.0 mg·L^-1 and 3.5 mg·L^-1 in regeneration of embryonic tips. The best inducing time was 16-20 h and the mean shoots per explant was 2.69 and 2.78, respectively.
Regeneration from cotyledonary nodes and embryonic tips of soybean ＂Peking＂ was studied. The disinfectant ways of the mercuric chloride and chlorine gas were used and the concentrations of 6-BA and 2, 4-D were studied in the culture medium The results showed that the sterilization effect of chlorine gas was better than that of mercuric chloride. The best concentration of 6-BA was 1.0 mg·L^-1 and the best concentration of 2, 4-D was 2.0 mg·L^-1 in the germinating medium. The number of buds of each explant was 3.56 and 2.98, respectively. The best concentrations of 6-BA and 2, 4-D were 3.0 mg·L^-1 and 3.5 mg·L^-1 in regeneration of embryonic tips. The best inducing time was 16-20 h and the mean shoots per explant was 2.69 and 2.78, respectively.
基金
Supported by Province Education Department Science and Technology Project (11531023)
Dean Fund Project of Key Laboratory of Soybean Biologyof Ministry of Education (SB 07A04)
