摘要
The follicle stimulating hormone beta-subunit （FSHβ） gene plays an important role in piglets. Marker-assisted selection （MAS） in conjunction with traditional selection methods is most effective for improving the piglets breeding traits. To find the new SNPs, the polymorphism of it in six pig breeds （Min, Landrace, Yorkshine, Duroc, wild boar, and wild boar × Landrace） was analyzed by using PCR-SSCP and was further compared with each other. Polymorphism was found and the sequencing results showed that there was one silent mutation on exon2 （C48T） and two mutations on exon3 （T422C and A514G）. Genotype distribution of FSHβ in Min and wild boar on exon3 was in accordance with Hardy-Weinberg Law.
The follicle stimulating hormone beta-subunit （FSHβ） gene plays an important role in piglets. Marker-assisted selection （MAS） in conjunction with traditional selection methods is most effective for improving the piglets breeding traits. To find the new SNPs, the polymorphism of it in six pig breeds （Min, Landrace, Yorkshine, Duroc, wild boar, and wild boar × Landrace） was analyzed by using PCR-SSCP and was further compared with each other. Polymorphism was found and the sequencing results showed that there was one silent mutation on exon2 （C48T） and two mutations on exon3 （T422C and A514G）. Genotype distribution of FSHβ in Min and wild boar on exon3 was in accordance with Hardy-Weinberg Law.
基金
Supported by National Key Technology R&D Program in the 11th Five Year Plan of China (2008BADB2B01)
