灵芝过氧化物同工酶和酯酶同工酶的研究

Electrophoretic studies on estease and peroxidase isozymes in Ganoderma sp.

目的：探讨利用同工酶技术对灵芝不同菌株的分类鉴定方法。方法：应用聚丙烯酰胺凝胶电泳法，分析比较过氧化物同工酶和酯酶同工酶酶谱的变化。结果：灵芝５个菌株间存在相似的酶带和相近的Ｒｆ值，亲缘关系极其相近，紫芝与其它菌株间酶谱差异较大，亲缘关系相对较远。

OBJECTIVE:To establish a method of electrophoresis and isozymography to classify and identify Ganoderma.METHOD:The changes in isoenzyme pattern were characterized by polyacrylamide gel electrophoretic (PAGE) and compared. RESULTS:The five strains of Ganoderma had some similar bands with common hereditary characteristics and close relationship. While isoenzyme zymogram of G. sinense was different from other strains with a distant relation. CONCLUSION:Estease isozyme analyses is an effective way to classify strain of Ganoderma.