摘要
目的：建立复方蛇床子胶囊中蛇床子素的含量测定方法，为该新产品提供质量控制标准。方法：以体积分数为５０％的乙醇为提取溶剂，超声振荡１５ｍｉｎ提取。色谱柱：ＳｈｉｍｐａｃｋＣＬＣＯＤＳ（１５０ｍｍ×４６ｍｍ，５μｍ），保护柱：自填国产ＹＷＧＣ１８Ｈ３７（２０ｍｍ×４６ｍｍ，１０μｍ），流动相：甲醇水四氢呋南（７０∶４０∶５），检测波长３１７ｎｍ，柱温３５℃。结果：方法回收率为９８５２％（ｎ＝６），精密度为ＲＳＤ＝１４４％（ｎ＝５）。结论：本实验建立的ＨＰＬＣ可以方便地测定复方蛇床子胶囊中的蛇床子素。
OBJECTIVE:To determine osthole in Fufangshechuangzi capsules for the quanlity control of this new preparation.METHOD:The samples were treated by ultrasonic shaking for 15min with 50% ethanol as extraction solvant. The HPLC method was penformed on Shimpack CLC ODS column(5μm,150mm×4.6mm) with a mobile phase of methanol water tetrahydrofuran(70∶40∶5).The flow rate of mobile phase was 1.0ml·min -1 .A guard column was packed with YWGC 18 H 37 (20mm×4.6mm,10μm).The UV detection was made at 317nm.The column tempreture was 35℃.RESULTS:The recovery of this method was 98.52%(n=6).The method reproducibility was RSD=1.44%(n=5).CONCLUSION:The method could be used to control the quality of Fufangshechuangzi capsules.
出处
《中国药学杂志》
CAS
CSCD
北大核心
1998年第12期745-748,共4页
Chinese Pharmaceutical Journal
