拟穴青蟹视神经节孕酮受体的免疫识别

Immunological Recognition of Progesterone Receptor in Optic Ganglia of the Mud Crab,Scylla paramamosain

研究表明,在甲壳类中可能存在类固醇的调控系统,然而核受体在进化的过程中是否依赖配体作用一直存在着争论.应用免疫印迹和免疫组织化学方法对拟穴青蟹(Scylla paramamosain)视神经节孕酮受体(PR)进行免疫识别.结果发现,拟穴青蟹视神经节中存在PR免疫阳性反应;其分子量约为70 ku,该物质广泛分布于视神经节的神经细胞核内.PR在拟穴青蟹视神经节的发现暗示着其类似于脊椎动物调节机制的存在;PR在神经细胞核中的定位表明其为核受体,很有可能在基因水平上参与拟穴青蟹视神经节的生理活动调节.

In the present study, the existence of steroids regulation system in crustaceans was found. However, it has been argued weather steroid receptors play a role in the regulation during the process of evolution. In the present study,by application of Western blot and immunohistochemistery methods, PR immunoreactivity was detected in the optic ganglia of Scylla paramamosain. As a result,PR immunoreactivity was found in the optic ganglia. And PR distributed extensively in the neuron nuclear of optic ganglia with a molecular weight of about 70 ku. The study suggested the existence of steroids regulation system in crustaceans like vertebrates. PR is a nuclear receptor which may mediate the physiological activities of optic ganglia via nuclear receptor in gene level.