pH色谱法测定灯盏花乙素离解常数

Determination of dissociation constant of scutellarin by thin-layer pH-chromatography and HPLC pH-chromatography

通过高效液相色谱(HPLC)和薄层色谱法(TLC)对灯盏花乙素离解常数进行测定方法研究.在薄层色谱方法中,利用待测组分比移值(Rf值)与固定相pH值的Rf-pH曲线方程;在高效液相色谱方法中,利用待测组分保留因子(κ值)与流动相pH值的κ-pH曲线方程,测定了灯盏花乙素的离解常数,并比较了HPLC和TLC法对灯盏花乙素离解常数的测定.HPLC与TLC法对灯盏花乙素离解常数的测定中κ-pH曲线和Rf-pH曲线均符合S型数理模型,该文首次报道了灯盏花乙素离解常数及测定方法.用所建立的方法分别测定了灯盏花乙素的离解常数并进行了比较,HPLC法准确度高,重现性好,方便简单,分析快速、准确.

Objective：To determine the dissociation constant of scutellarin by thin-layer pH-chromatography and HPLC pH-chromatography.Method：The dissociation constant of scutellarin is calculated respectively from the data of Rf-pH curves in the thin-layer pH-chromatography,and the retention factors-pH of mobile phase in the HPLC pH-chromatography.Results：The curves of the two methods showed to be suitable for sigmoidal fit.The dissociation constant of scutellarin and its determination were studied for the first time.Conclusion：Compared the thin-layer pH-chromatography with the HPLC pH-chromatography,the method of HPLC ph-chromatography is more repetitive,rapid and accurate.