人基因重组IFN-γ对滑膜细胞分泌TNF-α作用机制的研究

THE EFFECT OF INTERFERON γ ON THE SYNOVIAL CELL SECRETION OF TNF α

目的：研究ＩＦＮγ对滑膜细胞分泌ＴＮＦα的影响。方法：将滑膜用胶原酶消化分离后获得的原代单个滑膜细胞（ＤＳＳＣ），加入不同剂量的ＩＦＮγ，观察脂多糖（ＬＰＳ）存在或不存在下，不同时间（２４小时、４８小时、７２小时）滑膜细胞分泌ＴＮＦα的情况；并观察了ＩＦＮγ对培养的滑膜纤维母细胞样细胞（ＳＦＣ）在４８小时时分泌ＴＮＦα的影响。用ＥＬＩＳＡ双抗体夹心法来检测培养细胞上清液中ＴＮＦα的含量，用配对ｔ检验来统计结果。结果：不论ＬＰＳ存在与否，ＩＦＮγ对ＲＡ和ＯＡ滑膜ＤＳＳＣ细胞分泌ＴＮＦα无明显的影响，但ＩＦＮγ对ＲＡ的ＳＦＣ分泌ＴＮＦα有抑制作用（４８小时）。

Objective:To investigate IFN γ effect on synovial cell secretion of TNF α.Methods:Synovium of knee joints from 6 RA patients and 5 OA patients were digested by collagenase to prepare DSSC (digested synovial single cells).DSSC ability to excrete TNF α and IFN γ ability to influence its secretion were investigated.Observation of IFN γ effect on synovial fibroblast like cell of 3 RA patients was also made.ELISA method was used to detect the level of TNF α.Results:IFN γ have no effect on TNF α secretion of DSSC at different time (24h,48h and 72h),irrespective of LPS present or not;but IFN γ can inhibit the TNF α secretion of synovial fibroblast like cell.When IFN γ concentration is 300×10 3U/L, P <0 05 ( t test).Conclusion:These results indicate IFN γ may inhibit the proliferation of fibroblast like cells.A clue to the new strategy in the treatment of RA is perhaps provided.