兽用中草药制剂肠杆灵散的毒性试验

Toxicity Test of Herbal Preparation Changganling San

肠杆灵散(暂定名)是贵州省畜牧兽医研究所自主研发的防治鸡大肠杆菌病的中草药制剂,为了明确该制剂在禽病防治上的安全性,根据国家《中药天然药物长期毒性研究指导原则》,用昆明小白鼠按最大给药量(生药)0.4 mL/10 g进行急性毒性试验(MTD),均无明显的毒性反应。用大鼠(SD品系)按10 g/kg5、g/kg、2 g/kg设高、中、低3个给药剂量组,连续灌药5周后,以最大程度暴露其毒性,对其凝血系统指标、血液生化指标、各主要器脏病理组织学变化等检查,结果表明:除Cr具有一定的差异外,其他未见异常,各剂量组具有统计学意义的指标,在不同给药变化未见明显规律性,且与给药剂量未见显著相关性,从而推测该制剂无毒理作用。

Changganling San（interim name） is an herbal preparation independently researched and developed by Guizhou Institute of Animal Husbandry and Veterinary Science for controlling chicken colibacillosis.In order to make sure security of the preparation on controlling of poultry diseases,the toxicity test was carried out based on national Guiding Principle for Long-Term Toxicity Research of Herbal and Natural Drugs.There was no clear toxic reaction in acute toxicity test by maximum tolerance dosage（MTD） with 0.4mL/10g of crude drug to Kunming little white mice.Three dosage groups with 10g/kg,5g/kg and 2g/kg respectively were continuous drenched on rats（SD strain） for 5 weeks to expose the toxicity at utmost,and the indexes of blood coagulation system and blood biochemistry,and histopathological changes of the main viscera were examined.The result showed no abnormity except some difference of Cr.With index of statistical significance,all dosage groups were no clear regularities and no significant difference.Therefore,the preparation was speculated without toxicity.