母猪哺乳期蛋白限饲对子代血脂水平、肌内脂肪含量及H-FABP基因表达的影响

Effects of Protein Restriction in Sows During Lactation on Serum Lipids,the Content of Intramuscular Fat,and H-FABP mRNA Expression in the Filial Pigs

【目的】本试验旨在研究哺乳期母猪蛋白限饲对子代血脂水平、肌内脂肪含量及H-FABP基因表达的影响。【方法】选取胎次、体重相近(180±5kg)、经同期纯种繁育的民猪及长白猪妊娠母猪12头(民猪和长白猪各6头)。将每个品种母猪随机分为2组,每组3个重复,每个重复1头母猪。供试母猪妊娠期营养水平相同,哺乳期对照组日粮蛋白水平为18%,限饲组蛋白水平为9%。在子代猪断奶(28d)和出栏(180d)时测定血清中血脂水平、背最长肌中肌内脂肪含量及心脏脂肪酸结合蛋白(H-FABP)基因相对表达量。【结果】母猪哺乳期蛋白限饲显著提高了长白猪子代28d时血清总胆固醇(CHO)含量(P<0.05),对180d子代影响不显著;有提高长白猪子代28d及180d时血清甘油三酯(TG)、低密度脂蛋白(LDL),降低高密度脂蛋白(HDL)含量的趋势,但差异不显著;民猪限饲组子代28d及180d时血清中CHO、TG和LDL含量高于对照组,HDL含量低于对照组,但差异均不显著。母猪哺乳期蛋白限饲显著提高了长白猪子代28d时肌内脂肪含量及H-FABP mRNA相对表达量(P<0.05),对180d子代也有一定程度的提高,且有提高民猪子代28d和180d肌内脂肪含量及H-FABP mRNA相对表达量的趋势,但差异均不显著。【结论】母猪哺乳期蛋白限饲可对子代早期脂肪沉积产生不同程度的母体效应,但这种影响随着日龄的增加而减弱,存在补偿效应,且猪种间表现出一定的差异。

[Objective] The objective of this experiment was to evaluate the effects of maternal protein restriction during lactation on serum lipids, intramuscular fat and H-FABP mRNA expression in the offspring. [Method] Twelve （6 Landrace and 6 Mix pig） multi-parity sows with an average body weight of 180±5 kg were chosen for experiment, and they were purely mated to Landrace or Mix pig boars at identical estrus date respectively. Sows were allotted into two groups, with three replicates each consisting of one sow. All sows fed with a same composition diet in gestation, received a diet of either 18% or 9% protein during lactation. The serum lipids level, intramuscular fat content and H-FABP mRNA expression of longissimus muscle was determined in 28 d and 180 d offspring. [Result] The result showed that the serum CHO was significantly increased in protein restricted group of Landrace offspring at 28 d （P〈0.05）, but no obvious differences were found in 180 d pigs. No significant difference was presented in 28 d and 180 d offspring of Landrace, although there was a tendency of increase in the serum TG and LDL, and decrease in the HDL. The serum CHO, TG and LDL of protein restricted group was increased and the HDL was decreased compared to that of the control group, but no significant effect was found in 28 d and 180 d offspring of Min pig. The content of intramuscular fat and H-FABP mRNA expression in longissimus muscle was significantly increased in protein restricted group of Landrace offspring at 28 d （P〈0.05）, but no significant difference was observed in 180 d pigs. Maternal protein restriction during lactation presented a tendency to increase in the content of intramuscular fat and H-FABP mRNA expression in 28 d and 180 d offspring of Min pig, however, the influence was not significant. [ Conclusion] The results indicated that there was a difference in influence of maternal protein restriction on fat deposition in the offspring. The effect was decreased with the prolongation of time. The maternal effect showed some difference between breeds.