鸡白细胞介素-1β基因内含子1、2多态与部分免疫性状的相关性分析

Correlation Analysis Between Single Nucleotide Polymorphisms of Intron 1 and 2 of IL-1β Gene and Immune Traits of Chicken

【目的】以白细胞介素-1β(Interleukin-1β,IL-1β)基因为候选基因,研究其单核苷酸多态性(single nucleotide polymorphism,SNP),及其与部分免疫性状的关系。【方法】根据红色原鸡IL-1β基因序列设计引物一对,利用PCR-SSCP方法检测了如皋鸡、文昌鸡以及安卡鸡IL-1β基因内含子1、2的SNP,同时检测了56日龄的血清IL-1浓度、异嗜性粒细胞/淋巴细胞(heterophil/lymphocyte,H/L)值、绵羊红细胞(sheep red blood cell,SRBC)抗体滴度、新城疫(newcastle disease,ND)抗体滴度、禽流感(avian influenza,AI)抗体滴度5个免疫性状,并进一步分析了SNP位点与免疫性状的关系。【结果】共检测出6个SNP位点,分别为T107G突变、T130A突变、C143T突变、A208C突变、T241G突变及T274C突变。经关联分析,发现107、208、241及274位点存在显著的基因型效应(P<0.05或P<0.01),基因型组合对H/L值及ND抗体滴度两个性状上存在显著效应(P<0.05)。【结论】241位点突变型个体3个免疫性状均优于野生型,有望在鸡的抗病育种中筛选为新的标记位点。利用基因型组合选择效果优于对单个位点的选择。

[Objective] The SNPs oflL-1β gene and their correlations with some immune traits were tested. [Method] A pair of primers were designed as the sequence of IL-1β of red jungle fowls, and the SNPs of intron 1 and 2 of IL-1β gene in Rugao Chicken（RG）, Wenchang Chicken（WC）, and Anka Chicken（AK） were detected using PCR-SSCP. Meanwhile, concentration of IL-1, H/L value, SRBC antibody titer, ND antibody titer and AI antibody titer were detected at 56 days of age. Then, the correlation between SNP sites and immune traits were analyzed. [Results] Totally 6 SNP sites were detected at T107G, T130A, C143T, A208C, T241G and T274C. Furthermore, the mutation at 107, 208, 241 and 274 sites were found association with immune traits （P 〈0.05 or P〈0.01）, and some genotype combinations significantly affected H/L value and ND antibody titer （P〈0.05）. [Conclusion] The mutation at 241 site may be selected as a new candidate mark in disease resistance breeding because of its better performance in three immune traits than that of wild type chickens. Selecting breeder chickens by genotype combinations had more effect than by single genotype of SNPs.