阻塞性睡眠呼吸暂停低通气综合征及其相关高血压患者血浆还原型谷胱甘肽与氧化型谷胱甘肽和血清还原型辅酶Ⅱ与氧化型辅酶Ⅱ水平的变化

Changes of Plasma GSH/GSSG and NADPH/NADP+ Levels in Patients With OSAHS and OSAHAHT

目的观察阻塞性睡眠呼吸暂停低通气综合征(OSAHS)及阻塞性睡眠呼吸暂停低通气综合征相关高血压(OSAHAHT)患者血浆还原型谷胱甘肽(GSH)、氧化型谷胱甘肽(GSSG)及血清还原型辅酶Ⅱ(NADPH)、氧化型辅酶Ⅱ(NADP+)水平。探讨OSAHS患者氧化还原态的变化。方法选择OSAHS患者39例,其中无并发症的OSAHS患者20例,OSAHAHT患者19例,;健康对照组19例,应用比色法和酶联免疫吸附法测定血浆GSH、GSSG,血清NADPH、NADP+水平。结果3组受检者血浆GSH、GSSG及血清NADPH、NADP+水平比较,差异均有统计学意义(P<0.01),其中OSAHS患者与对照组比较,OSAHAHT与OSAHS患者组比较,血浆GSH/GSSG、血清NADPH/NADP+比值均降低(P<0.05),并且与反映睡眠呼吸暂停严重程度的指标均呈负相关(P<0.05)。结论OSAHS患者存在GSH/GSSG、NADPH/NADP+比值的变化,且与OSAHS的病情严重程度相关;氧化还原态的变化在OSAHAHT患者中更明显。

Objective To determine the levels of plasma reduced glutathione （GSH）,oxidized glutathione（GSSG） and serum reduced coenzyme II （NADPH）,oxidized coenzyme II（NADP^＋） to explore the redox state changes of OSAHS in patients with obstructive sleep apnea-hypopnea syndrome（OSAHS）without complications and in those with obstructive sleep apnea-hypopnea syndrome associated hypertension（OSANHAHT）.Methods The levels of plasma GSH,GSSG and serum NADPH,NADP^＋ were determined by spectrophotography and enzyme linked immunosorbent assay （ELISA） in groups OSAHS （OSAHS patients without complications,n=20）,OSAHANT （n=19） and control （n=19）.Results There was significant difference in levels of plasma GSH,GSSG and serum NADPH,NADP between 3 groups （P〈0.01）.The levels of plasma GSH/GSSG and serum NADPH/ NADP^＋ were lower in OSAHS group than in control and lower in PSAHAHT group than in OSAHS group（P〈0.05）,and negatively correlated with AHI,CT90%,ODI and ArI.Conclusion The changes of GSH/GSSG and NADPH/ NADP^＋,existing in OSAHS patients,are correlated with the severity of OSAHS;redox state changes are more pronounced in OSAHAHT patients.