水通道蛋白-8在羊水过少人胎盘、胎膜中的表达

Expression of Aquaporin-8 in Human Oligohydramnios Placenta and Fetal Membranes

目的了解水通道蛋白-8(aquaporin-8,AQP-8)在羊水过少人胎盘、胎膜中的表达。方法随机选择2008年12月至2009年1月在广东省深圳市福永医院妇产科行选择性剖宫产孕妇16例为研究对象,满足单胎、足月、无妊娠合并症、未临产、无胎儿窘迫及胎儿畸形条件。将产前超声监测羊水深度<8cm的孕妇纳入研究组(羊水过少组,n=6),其余孕妇纳入对照组(n=10)(本研究遵循的程序符合深圳市福永医院人体试验委员会所制定的伦理学标准,得到该委员会批准,分组征得受试对象本人的知情同意,并与之签署临床研究知情同意书)。两组患者年龄、妊娠情况及妊娠结局等比较,差异无显著意义(P>0.05)。采用免疫组织化学链霉亲和素-生物素-过氧化物霉(streptavidin enzyme complex,SABC)法检测两组胎盘和胎膜组织标本中水通道蛋白-8表达水平。结果水通道蛋白-8在人胎盘及胎膜的羊膜上皮细胞均有表达,对照组胎膜中水通道蛋白-8表达明显低于研究组,且差异有显著意义(P<0.05)。结论水通道蛋白-8在人羊水过少的胎盘、胎膜中表达显著减少,提示水通道蛋白-8在母、胎液体交换及羊水膜内吸收途径中,可能发挥重要作用。

Objective To detect aquaporin-8（AQP-8） expression in human oligohydramnios placenta and fetal membranes. Methods From December 2008 to January 2009, placenta and fetal membranes samples were obtained from 6 women with oligohydramnios （study group） and 10 women with normal amniotic fluid volume（control group）. All of them met the following conditions： Single birth, full term, not in labor, and without pregnancy complications, fetal distress and fetal malformations. All the 16 cases underwent elective cesarean section in department of Obstetrics and Gynecology, Fuyong Hospital of Shenzhen. Informed consent was obtained from all participants. There had no significant difference in age, pregnancy situations, and pregnancy outcome（P〉0.05）. Aquaporin 8 expression in the tissue samples was detected by immunohistochemical streptavidin enzyme complex （SABC） method. Results Aquaproin-8 expression was detected in both human oligohydramnios placenta and fetal membranes. Aquaproin 8 expression of control group was significantly lower than that of study group （P〈0. 05）. Conclusion Aquaproin 8 expression has a significant reduction in human oligohydramnios placenta and fetal membranes, and it may play an important role in the maternal-fetal fluid exchange and absorption of membrane channels.