大肠埃希菌、肺炎克雷伯菌产超广谱β-内酰胺酶检测及药敏分析

Detecting and analyzing antibiotic susceptibility of extended - spectrum β - lactamase - producing in E. Coli and Klebsiella pneumoniae

目的统计分析肠杆菌科中的大肠埃希菌和肺炎克雷伯菌产超广谱＇李云。－内酰胺酶（ESBLs）检出率和药敏情况，为临床提供合理使用抗生素用药依据。方法本文收集笔者所在医院2006年1月－2009年9月门诊和住院患者各种临床标本检出的大肠埃希菌200例，肺炎克雷伯菌99例，统计分析产ESBLs阳性率及药敏试验。结果产ESBLs大肠埃希菌、肺炎克雷伯菌的阳性检出率分别为69．5％和48．5％，其药敏：对阿米卡星、哌拉西林／他唑巴坦、头孢哌酮／舒巴坦的敏感率分别为：84．9％和89．6％、84．O％和75．0％、66．2％和79．2％。结论产ESBLs菌的检测，对指导临床用药有重要的意义。

Objective Analyzing the positive detectable rate and antibiotic susceptibility of extended - spectrum β - lactamase - producing in E. Coli and Klebsiella pneumoniae, provides clue for intelligent use of antibiotic in clinic. Methods 200 strains of E. Coli and 99 strains of Klebsiella pneumoniae isolated from clinical samples of outpatients and inpatients in our hospital from January 2006 to September 2009 were collected in this paper, the positive detectable rate and drug sensitivity of ESBLs -producing enterobacteria were analyzed with Statistical methods. Results The positive detectable rate of ELBS - producing in E. Coli and Klebsiella pneumoniae was 69.5% and 48.5% . There were 3 antimicrobials to which the sensitive rates of ELBS - producing in E. Coli and Klebsiella pneumoniae ,to Amikacin being 84.9% and 89.6%, to piperacillin - tazobactam being 84% and 75 %, to Cefoperazone - Sulbactam being 66.2% and 79.2% . Conclusion Detecting ESBLs -pro ducing enterobaeteria has important meaning for clinical aoolication.