锥栗叶片两种提取蛋白方法的比较

Effect of Two Methods of Protein Extraction from leaves of Castanen henryi Rehd & Wils

比较三氯乙酸/丙酮沉淀法、酚柚提法两种方法对锥栗叶片总蛋白的提取效果。结果表明:酚抽提法获得的蛋白质产率高、纯度高;且其提取蛋白质所得电泳图谱背景浅、拖尾轻、蛋白点分散且性状规则,适合锥栗叶片蛋白质的提取。试验为锥栗胚胎发育期蛋白质组学研究提供参考,也对其他干扰物质含量高的植物组织蛋白提取有一定的参考价值。

The effect of the general protein extraction ofCastanen henryi Rehd ＆ Wils leaves between trichloroaeetic acid- acetone precipitation method（TCA method） and phenol isolation method（phenol method） has been compared. The results were as follows： the yield ratio and purity of the protain by phenol method were better than those by TCA method; phenol isolation method could obtain a low-background graph with few blurred and smearing spots; besides, the spots scatterd and had regular shape, and thus this method is optimal for extracting leaves protein from Castanen henryi Rehd ＆ Wils. As a result, the test results can provide reference for the proteomics research of Castanen hemyi Rehd ＆ Wils embryo during the developing period and have significant value for guiding the protein extraction of many other plants containing various interfering elements.