萱草再生体系技术建立的研究

Establishment of Regeneration System of Hemerocallis fulva

以萱草的根茎为材料,进行愈伤组织诱导和分化,试管苗的生根、移栽和移植的研究,建立萱草再生体系技术。结果证明:MS+BA0.4mg/L+NAA0.1mg/L+2,4-D0.1mg/L是愈伤组织诱导培养的理想培养基;MS+NH_4H_2PO_450mg/L+BA1.0mg/L+NAA0.1mg/L是愈伤组织增殖继代培养的理想培养基;MS+AgNO_30.5mg/L+GA_30.5mg/L+BA0.5mg/L+NAA0.1mg/L是愈伤组织分化培养的理想培养基;1/2MS+NAA0.1mg/L+IAA0.2mg/L是不定芽生根培养的理想培养基。在河沙中试管苗易移栽成活;移植到花坛中的试管苗根系发达、生长旺盛。

The study was mainly to probe an efficient way through tissues culture and rapid propagation of Hemerocallis fulva. The rhizome was taken as explants.The main results are as follows：The optimum medium was MS＋BA0.4mg/L＋NAA0.1mg/L＋2,4-D0.1mg/L for inducing callus; the optimum culture medium of proliferating callus was MS＋NH4H2PO450mg/L＋BA1.0mg/L＋NAA0.1mg/L; the optimum culture medium of differentiating callus was MS＋AgNO30.5mg/L＋GA30.5mg/L＋BA 0.5mg/L＋NAA 0.1mg/L; the optimum culture medium for rooting was 1/2MS＋NAA0.1mg/L＋IAA0.2mg/L.The transplant medium with riversand is optimal for Hemerocallis fulva. Seedling transferred from test tubes grew well.