大肠癌组织酶标志物研究

Study on the Enzyme Markers in Colorectal Cancer Tissues

目的：探讨大肠癌的发病机理及早期诊断的酶标志物。方法：采用酶学方法测定大肠癌及近心端和远心端癌旁组织中鸟氨酸脱羧酶（ＯＤＣ）、γ－谷氨酰转肽酶（γ－ＧＴ）、乳酸脱氢酶（ＬＤＨ）活性及ＬＤＨ同工酶谱。结果：（１）大肠癌组织中ＯＤＣ、γ－ＧＴ及ＬＤＨ活性均显著高于癌旁正常组织；（２）大肠癌组织中ＬＤＨ５同工酶及ＬＤＨ４＋ＬＤＨ５／ＬＤＨ１＋ＬＤＨ２的比值也显著升高，ＬＤＨ同工酶谱向Ｍ型转移。结论：肠组织中ＯＤＣ、γ－ＧＴ、ＬＤＨ活性升高，ＬＤＨ同工酶谱向Ｍ型转移与大肠癌的形成有关，提示ＯＤＣ。

Objective:To Study the pathogenesis and the diagnostic markers of the colorectal cancer.Methods:Activity of ornithine decarboxylase(ODC),γ glutamyl transpeptidase(γ GT),Lactate dehydrogenase(LDH),and LDH isoenzyme pattern in the colorectal cancer and in the adjacent tissues were assayed by using spectrophotometric procedures and agarose electrophoresis,respectivily. Results: (1)The activity of ODC, γ GT, LDH in colorectal cancer tissues was about 4.0,3.5 and 1.5 times higher than that in adjacent noncancerous tissues,respectively;(2)LDH isoenzyme pattern in cancer tissues,was also significantly different from that in the control.The Persentages of LDH 5 were very much increased;but the LDH 1 were much decreased.Conclution:the increased ODC,γ GT and LDH activity,and alteration of LDH isoenzyme pattern was related to the pathogenesis of colorectal cancer.