摘要
目的研究辛伐他汀对人脐静脉内皮细胞的三磷酸腺苷结合盒转运体A1(ABCA1)表达的影响。方法体外培养人脐静脉内皮细胞,并分别与BSA(对照组)及1、10μmol/L的辛伐他汀组温育24h。采用逆转录聚合酶链反应、流式细胞技术分别检测人脐静脉内皮细胞的ABCA1的mRNA及蛋白表达水平。结果随着给药浓度的增加,人脐静脉内皮细胞的ABCA1表达逐步升高,与对照组比较差异有统计学意义(P<0.05)。结论辛伐他汀的调脂途径可能与其上调人脐静脉内皮细胞的ABCA1表达有关。
Objective To study the effect of simvastatin on the expression of ATP-binding cassette transporter A1(ABCAl)in human umbilical vein endothelial cells(HUVECs).Methods The HUVECs were cultured in vitro and incubated with simvastatin at different concentrations(0μmol/L,1μmol/L,10μmol/L)for 24h,and ABCA1 expression levels were determined by RT-PCR and flow cytometry.Results Simvastatin increased ABCA1 expression in HUVEC in a concentration-dependent manner(P0.05).Conclusion Simvastatin increases ABCA1 expression in HUVECs,which is related to its lipid lowering properties.
出处
《广东医学》
CAS
CSCD
北大核心
2010年第4期416-418,共3页
Guangdong Medical Journal
基金
广东省科技计划资助项目(编号:2007B031507001)