大承气汤对内毒素损伤溶酶体的保护作用

Protective Effect of Dachengqi Decoction on Lysosomes Induced by Endotoxin

目的：探讨大承气汤保护肠道屏障功能、治疗内毒素血症的理论。方法：体外实验选用大鼠肝溶酶体，体内实验选用内毒素所致家兔全身性施瓦茨曼反应（ＧＳＲ）作为弥漫性血管内凝血（ＤＩＣ）模型，并给予不同处理，借测定溶酶体标志酶——酸性磷酸酶（ＡＣＰ）活性，以判断溶酶体膜是否完整及大承气汤对其保护作用。结果：模型组动物肝、肠溶酶体ＡＣＰ活性明显升高，而大承气汤治疗组则与正常对照组相似。结论：大承气汤能够稳定肝、肠溶酶体膜，减少溶酶外逸。

Objective:To study the theoretical basis of Dachengqi decoction in protecting the gut barrier and treating endotoxemia.Methods:Wistar rats(n=6) were killed and livers were removed.Liver homogenates were made by routine assay,isolating lysosomes from hepatic homogenates by differential centrifugation.In vivo,endotoxin was given to rabbits intravenously twice,with an interval of 24 hours to induce generalized Shwartzman reaction(GSR).These animals were served as disseminated intravascular coagulation(DIC) models and given various treatments.Acid phosphatase(ACP) in lysosomes were measured by 4 aminoantipyrine method.Results:In Dachengqi decoction group the lysosomal ACP activity was lowered markedly than in the DIC model group;when compared with the control group,there was no difference.In order to exclule the direct action of the medicine on ACP activity another experiment was carried out,namely different concentrations of Dachengqi decoction and endotoxin acting on calibrated acid phosphatase.The results showed simularities of ACP activity on either group( P >0.05).Conclusion:Dachengqi decoction could inhibit lysosomal damage induced by endotoxin,preserve intracellular organelle and the gut barrier function,thus minimizing endotoxin injury to the organism.