摘要
目的探讨外周血中T-bet和GATA-3基因的表达在再生障碍性贫血(aplastic anemia,AA)中发病机制及意义。方法选择23例AA患者为再障组,其中重型再障(severe aplastic anemia,SAA)13例,轻型再障(mediate aplastic anemia,mAA)10例,再选择22例健康体检为对照组,对纳入对象采用流式细胞术检测其外周血Th1和Th2,同时运用RT-PCR技术检测外周血单个核细胞(PBMCs)中转录因子T-bet和GATA-3 mRNA基因表达。结果与对照组相比,再障组的lg(T-bet/β-actin)和Th1(%)升高,差异有统计学意义(P<0.05、P<0.01),lg(GATA-3/β-actin)和Th2(%)降低,差异有统计学意义(P<0.05、P<0.05);其中SAA组的lg(T-bet/β-actin)、lg(GATA-3/β-actin)和Th1(%)、mAA组的lg(T-bet/β-actin)、Th1(%)相比对照组明显升高(P<0.05、P<0.01、P<0.01、P<0.05、P<0.05);SAA组的Th2(%)、mAA组的lg(GATA-3/β-actin)和Th2(%)相比对照组显著降低(P<0.05、P<0.01、P<0.05)。结论T-bet和GATA-3表达异常在AA发生、发展过程中发挥重要作用,可能机制为增强Th1细胞功能,抑制Th2细胞功能,导致患者免疫功能异常,最终引起再障发生。
Objective To investigate the pathogenic role of T-bet and GATA-3 mRNA expression in aplastic anemia（AA）.Methods Twenty-three patients with AA（AA group） and 22 healthy people（control group） were included.Flow cytometry was used to detect the expression of Th1 and Th2 in peripheral blood and RT-PCR for quantifying the expression of transcription factor T-bet and GATA-3 mRNA.Results Compared with the control group,the expression of Th1 and T-bet mRNA in the AA group significantly increased（P〈0.01,P〈0.05）,while the expression of Th2 and GATA-3 mRNA decreased（P〈0.05）.Conclusion Abnormal expression of T-bet and GATA-3 plays an important role in the development and progression of AA,with the possible mechanism of enhancing Th1 cells and inhibiting Th2 cells.
出处
《广东医学》
CAS
CSCD
北大核心
2010年第3期290-292,共3页
Guangdong Medical Journal
基金
国家自然科学基金资助项目(编号:30600837)
