肢体缺血再灌注时红细胞内MDA、血浆中TXB_2和6-Keto-PGF_(1α)含量的变化

THE ALTERATION OF MDA LEVEL IN ERYTHROCYTE AND TXB 2 AND 6-keto-PGF 1α LEVEL IN PLASMAS AFTER REPERFUSION OF ISCHEMIC LIMB

将２１例择期下肢手术病人随机分为非缺血对照组、缺血再灌注组，以观察肢体缺血再灌注时红细胞内丙二醛（ＭＤＡ）、血浆中血栓素Ｂ２（ＴＸＢ２）和６－酮－前列腺素Ｆ１α（６－Ｋｅｔｏ－ＰＧＦ１α）含量的变化。结果发现，肢体缺血再灌注１０ｍｉｎ后，红细胞内ＭＤＡ显著升高，血浆中ＴＸＢ２及ＴＸＢ２／６－Ｋｅｔｏ－ＰＧＦ１α的比值无明显变化；再灌注２０ｍｉｎ后，红细胞内ＭＤＡ、血浆中ＴＸＢ２以及ＴＸＢ２／６－Ｋｅｔｏ－ＰＧＦ１α比值均显著升高。研究提示肢体缺血再灌注后，红细胞脂质过氧化加强先于ＴＸＡ２－ＰＧＩ２平衡失调。

The alteration of Malonaldehydic acid (MDA) level in erythrocyte and thromboxane B2 (TXB 2) and 6-keto-PGF 1α level were observed in plasmas of 21 patients for selected low limb operation, who were devided into two subgroups, non-ischemic and ischemia-reperfusive. Results: after 10 minutes of reperfusion of ischemic limb, MDA level in erythrocyte elevated markedly, while no significant changes were found of the TXB 2 level and the ratio of TXB 2 to 6-keto-PGF 1α in plasma; after 20 minutes, both of the above results showed significant elevation. The present data suggest that reperfusion of ischemic limb intensified lipid peroxidation of erythrocyte, which proceeds the unbalance between TXA 2 and PGI 2.