化学发光法测定二氢青蒿素

DETERMINATION OF DIHYDROARTEMISININ BY CHEMILUMINESCENCE METHOD

二氢青蒿素与鲁米诺在碱性条件下不需过氧化氢就能直接发生化学发光反应，氯化血红素对这一新的体系同样具有强烈的催化作用，运用这一新的发光体系建立了灵敏度高、特异性强、操作方便的测定抗疟新药二氢青蒿素的化学发光新方法，线性范围为０～３０００ｎｇ／ｍｌ，线性方程为Ｙ＝１．１４６Ｘ＋２．９３（０～１００ｎｇ／ｍｌ），ｒ＝０．９９４；Ｙ＝２．１７Ｘ－１３９（１００～３０００ｎｇ／ｍｌ），ｒ＝０．９９９，检出限为１．４ｎｇ／ｍｌ，变异系数为３．３％。文中提示二氢青蒿素中的过氧键是鲁米诺发光反应所需的超氧自由基的来源。

Without hydrogen peroxide dihydroartemisinin reacted with luminol under alkaline condition. The C.L signal ws generated from the reaction which was catalyzed by hemin. So a new sentisitive,specifical and simple chemiluminescence method of determination of antimalarial agent dihydroartemisinin was developed .The linear range is 0～3000ng/ml.The detection limit is 1.4ng/ml. The endoperoxide moiety on dihydroartemisinin is implicated as a contributing source of superoxide radicals required for the light-producing reaction with luminol.