络气郁滞证大鼠动物模型的建立和评价

Establishment and Evaluation of Rat Animal Model with Collateral Qi Stasis

目的建立络气郁滞证大鼠动物模型并进行评价。方法对大鼠慢性束缚6周建立络气郁滞证模型,观察模型大鼠的精神、饮食量、体质量、蔗糖水摄取量,血清皮质酮(CORT)、血浆内皮素(ET),血清5羟色胺(5-HT)、NO以及主动脉组织内皮细胞超微结构的变化。结果络气郁滞证模型表现为精神萎靡,反应迟钝,摄食量减少,体质量增长缓慢,蔗糖水摄取量、摄食量及体重均明显低于对照组(P<0.05或P<0.01);模型组大鼠血浆ET升高、血清CORT升高,5-HT、NO降低(P<0.05或P<0.01);内皮细胞超微结构发生明显改变。结论利用慢性束缚法6周,通过观测大鼠体质量、摄食量、蔗糖水摄取量、CORT、ET、NO、5-HT的变化,初步建立络气郁滞证大鼠动物模型,制作方法简单,评价指标客观,为脉络血管系统病的研究提供良好的实验基础。

Objective To establish and evaluate the rat animal model with collateral qi stasis.Methods To establish animal model with collateral qi stasis in 6 weeks by chronic constraint. To observe the changes of consciousness,appetite,body weight,the intake of cane sugar water,and the levels of CORT,ET,NO,5-HT.The ultramicrostructure change of aorta was observed by transmission electron microscope.Results The animal model rats showed deprementia,dull,no appetite,increasing slow of body weight,the cane sugar water intake was lowered significantly（P〈0.01 or P〈0.05）,the levels of CORT,HCY,ET were increased significantly（P〈0.01 or P〈0.05）,and the levels of 5-HT,NO were lowered significantly（P〈0.01 or P〈0.05）.The ultramicrostructure of aorta changed obviously.Conclusion The animal model with collateral qi stasis is established with chronic constraint.The method has been succeed through observation the changes of body weight,cane sugar water intake,appetite,CORT,ET,NO,5-HT,and ultramicrostructure of aorta.The method is easy and the evaluated indexes are objective,which provide the good experiment foundation for the study of vessel-collateral and vascular system disorders.