超声波协同提取鱼腥草黄酮及其抗氧化性

Extraction of flavonoid from Houuuynia cordate by ultrasound and its anti-oxidation

研究了超声波协同乙醇提取不同部位鱼腥草黄酮的最佳工艺条件,并测定黄酮提取物对DPPH.、羟基自由基和超氧自由基的抑制效果。结果表明:鱼腥草叶中黄酮含量最高,在固液比1∶30,85%乙醇浓度,65℃下提取40min,黄酮提取率为2.36%。鱼腥草黄酮对DPPH.自由基活性、.OH自由基和超氧O2-.自由基的清除率可达72.8%、70.8%和69.8%,表明鱼腥草黄酮是一种天然有效的自由基清除剂。

The optimal process conditions of ethanol extraction for flavonoid in different parts of Houuuynia cordate assisted by ultrasound was researched,inhibition effects of flavonoid extraction on DPPH,hydroxyl radical,superoxide radical were also studied. The results showed that the flavonioid content in the leaves was the highest,and under the optimal conditions（the ratio of solid to liquid is 1∶30,alcohol 85% at 65 ℃ for 40 min）,the yield of flavonoid was 2.36%. H.cordate flavonoid possessed strong scavenging activity on DPPH,hydroxyl radical,superoxide radical with corresponding maximum scavenging rate 72.8%,70.8% and 69.8%,suggesting that it would be an effective natural free radical scavenger.