苯基荧光酮-SDBS-β-CD-荧光猝灭法测定微量Mn(Ⅱ)

Fluorescence quenching method for determining trace manganese(Ⅱ) with phenylfluorone in the presence of SDBS and β-CD

在pH7.4乙酸-乙酸钠介质中,有锰(Ⅱ)存在时,反应体系苯基荧光酮(PF)、β-环糊精(β-CD)及十二烷基苯磺酸钠(SDBS)所产生的荧光,由于锰(Ⅱ)与PF反应生成了配合物而产生猝灭现象,而且荧光强度的减弱程度与锰(Ⅱ)的质量浓度在0.12 mg/L以内呈线性关系。荧光检测体系在激发波长373 nm和发射波长534 nm条件下进行。基于上述事实,提出了测定微量锰的荧光猝灭法。应用此法测定自来水中的Mn(Ⅱ)的含量,结果令人满意。

It was found that the fluorescence emitted by the reaction system of phenylfluorone（PF）,SDBS and β-CD in a HAc-NaAc buffer medium of pH 7.4 was quenched by the presence of manganese ions due to the formation of a coordination complex between Mn（Ⅱ） and PF.The magnitude of decrease in fluorescence intensity（ΔF） was found to be in a linear relationship with the concentration of manganese ions in the range within 0.12 mg/L of solution,when the fluorescence measurement was made at the wavelengths of 373 nm and 534 nm.Based on these facts,a fluorescence quenching method for the determination of trace manganese was proposed.The method has been applied to the direct determination of trace manganese（Ⅱ） in tap water samples with satisfactory results.