紫外分光光度法测定海滩牵牛中总香豆素类成分的浓度

Determination of Total Content of Coumarin in Ipomoea Stolonifera by Ultraviolet Spectrophotometry

目的：测定草药海滩牵牛（IS）药材中总香豆素浓度。方法：采用紫外分光光度法，检测波长320nm，以伞形花内酯为对照品。结果：IS在2-32μg/mL质量浓度范围内，对照品吸光度和浓度线性关系良好（r=0．999）。平均回收率99．31％，相对标准偏差=1．88。结论：该法简便、可靠，为有效控制Is药材质量奠定了一定的基础。

Objective： To determine the total content of coumarin in ipomoea stolonifera. Method： The cournarin in ipomoea stolonifera was detected at 320 nm by ultraviolet speetrophotometry. Results： There was a good linear relationship within the range of 2-32 μg/mL（r=0.999）. The average recovery was 99.31% （ n=3）with relative standard of 1.88%. Conclusion： This method is rapid, reproducible, accurate and can be used for detecting the total content of coumarin in ipomoea stolonifera.