摘要
目的:测定草药海滩牵牛(IS)药材中总香豆素浓度。方法:采用紫外分光光度法,检测波长320nm,以伞形花内酯为对照品。结果:IS在2-32μg/mL质量浓度范围内,对照品吸光度和浓度线性关系良好(r=0.999)。平均回收率99.31%,相对标准偏差=1.88。结论:该法简便、可靠,为有效控制Is药材质量奠定了一定的基础。
Objective: To determine the total content of coumarin in ipomoea stolonifera. Method: The cournarin in ipomoea stolonifera was detected at 320 nm by ultraviolet speetrophotometry. Results: There was a good linear relationship within the range of 2-32 μg/mL(r=0.999). The average recovery was 99.31% ( n=3)with relative standard of 1.88%. Conclusion: This method is rapid, reproducible, accurate and can be used for detecting the total content of coumarin in ipomoea stolonifera.
出处
《汕头大学医学院学报》
2010年第1期5-6,共2页
Journal of Shantou University Medical College
基金
广东省科技计划资助项目(2008B030301240)
广东省中医药局资助项目(2008162)
国家自然科学基金资助项目(3097-3922)
广东省自然科学基金团队资助项目(9351503102000000)
关键词
海滩牵牛
总香豆素
紫外分光光度法
浓度
测定
ipomoea stolonifera, total coumarin, ultraviolet spectrophotometry, content, determination