几种修饰剂共价修饰L-门冬酰胺酶降低其抗原性的对比研究

Influences of Modifiers and Methods of Modification on Activity and Antigenicity of 1-Asparaginase

选用单甲氧基聚乙二醇、右旋糖苷、磺化右旋糖苷,肝素及小分子醋酸酐对酶分子进行了共价修饰,研究了不同修饰剂对酶分子的酶活力及其抗原性的影响。结果表明,大分子,特别是单甲氧基聚乙二醇的降低抗原性效果很好,但活力损失较大;修饰剂分子量越大,酶表面氨基修饰程度越高,解除抗原性效果越好。

Small and macromolecular modifiers and different methods were used for modification of E. coli L-asparaginase to reduce the antigenicity.The results showed that the macromolecular modifiers are better than that of small molecular modifier acetic anhydride for reduction of antigenicity.A comparison of the modified L-asparaginase by PEG, dextran,dextran sulfate and heparin showed that there is a relationship between the rate of modified free amino groups and the reduction of antigenicity.