甲磺酸加替沙星-Al(Ⅲ)荧光体系的研究及应用

Study on Fluorescence System of Gatifloxacin Mesilate-Aluminum and Its Analytical Application

[目的]研究铝（Ⅲ）-甲磺酸加替沙星荧光体系测定微量铝（Ⅲ）方法的建立与应用。[方法]在C6H4CO2HCO2K-NaOH介质中,铝与甲磺酸加替沙星形成络合物使甲磺酸加替沙星的荧光强度（ΔF）显著增敏,由此建立了测定铝的新方法,并研究不同溶液酸度、表面活性剂、甲磺酸加替沙星用量、试剂加入顺序对该体系ΔF的影响。[结果]荧光光谱图显示,该体系的最大激发波长λex=365 nm,最大发射波长λem=470 nm。溶液的酸度对该体系的ΔF有较大影响,pH值在5.50～6.50时可使该体系的增敏作用达到最大且较稳定。加入表面活性剂对ΔF均无较明显的影响。该体系中铝的浓度在1.58×10^-8～4.24×10^-6mol/L范围内与ΔF=F-F0具有良好的线性关系,最低检出限为8.36×10^-9mol/L,回收率为99.4%～101.4%。[结论]该方法用于测定食品中微量铝的含量可获得灵敏度较高和选择性较好的结果。

[Objective] The purpose was to study the establishment and application of the method for determining the microelement aluminum by using the fluorescence system of gatifloxacin mesilate-Aluminum.[Method] In the medium of C6H4CO2HCO2K-NaOH,the aluminum and gatifloxacin mesilate were formed into a complex which enhanced the sensitivity of fluorescence intensity（ΔF）of gatifloxacin mesilate,thus establishing a new method for the determination of aluminum.The effects of different solution acidity,surfactant,gatifloxacin mesilate dosage and reagent adding order on ΔF of this system were studied.[Result] The fluorescence spectrogram showed that the maximum excitation wavelength was λex = 365 nm and the maximum emission wavelength was λem=470 nm.The solution acidity had greater effect on ΔF and pH at 5.50-6.50 could made the sensitivity enhancing action of this system reaching maximum and being more stable.Adding surfactant had no obvious effect on ΔF of this system.In the system,the aluminum in 1.58×10^-8-4.24×10^-6 mol/L could get a good linear relationship with Δ F = F-F0,with the minimum detection limit of 8.36 × 10^-9mol/L,and the recovery rate of 99.4%-101.4%.[Conclusion] The new method used in determining the content of aluminum in food could get the results of higher sensitivity and better selection.