小麦TP突变体幼苗在光胁迫条件下的基因表达差异

Expression Differences in TP Mutation of Wheat under Light Stress

[目的]利用RAPD RT-PCR的方法研究小麦TP突变体幼苗在光胁迫下基因的表达差异。[方法]以100条随机引物筛选正常光照下小麦TP突变体幼苗和光胁迫下的小麦TP突变体幼苗。[结果]3条引物能扩增出差异性条带,其中TpS23在正常光照下特异性表达,而TpS102和TpS107在光胁迫条件下特异性表达,通过NCBI中的Blastn和Blastx比对发现,TpS23和乌拉尔图小麦的质体Acc1基因同源,TpS102与水稻的Ty3-gypsy反转录转座子同源,而TpS107是一个未知序列。[结论]该研究为克隆这3个基因的全序列和分析这些基因的功能奠定了基础。

[Objective]The aim was to study on the differential expression gene in TP mutation of wheat under light stress with RAPD RT-PCR method.[Method]The seedling of TP mutation in wheat under normal light and light stress were screened using 100 random primers.[Result]The results indicated that the expanded products of three primers showed specific character among the normal light line and light stress line,in which,Tps23 expressed only in the normal light line,while,Tps102 and Tps107 specific expressed in the light stress line.Sequence alignments use blastn and blastx showed that Tps23 and Acc1 gene in Triticum urartu shared high similarity in nucleotide level,and the Similarity of amino acid sequence among Tps102 and Ty3-gypsy retrotransposons were 41%.But the Tps107 was an unknown sequence.[Conclusion]This study will provide the basis for clone the full-length and characterize those three genes in TP mutation of wheat.