不同药物对温氏附红细胞体体外杀灭效果研究

Study on Killing Effects of Different Medicines on Eperythrozoon wenyonii in Vitro

[目的]筛选对温氏附红细胞体体外杀灭具有最佳效果的药物。[方法]以RPMI-1640为基础培养液,加入30%犊牛血清,选用盐酸土霉素、恩诺沙星、磺胺对甲氧嘧啶、硫酸庆大霉素、磷酸伯氨喹啉、盐酸咪唑苯脲、贝尼尔7种药物,在37℃、5%CO2的环境下,进行温氏附红细胞体体外药物杀灭试验。[结果]磷酸伯氨喹、盐酸咪唑苯脲对牛附红细胞体的体外杀灭效果最佳,当培养液药物浓度为2 000μg/ml,药物与附红细胞体作用6 h时,红细胞染虫率显著下降到30%以下。盐酸土霉素、恩诺沙星、贝尼尔和磺胺对甲氧嘧啶对附红细胞体均有一定作用。硫酸庆大霉素具有较强抗菌作用,但对牛附红细胞体病无效。[结论]该研究为临床选择抗附红细胞体病的合适药物提供理论依据。

[Objective] The study aimed to screen the medicines that had the optimum killing effects on Eperythrozoon wenyonii in vitro.[Method] With RPMI-1640 as the basic medium,adding 30% calf serum,the 7 medicines including oxytetracycline hydrochloride,enrofloxacin,sulfonamides methoxy-pyrimidine,gentamicin sulfate,primaquine phosphate morpholine,zmizol and bernier were selected to make for the medicine killing test on E.wenyonii in vitro under 5% CO2 environment at 37 ℃.[Result] The primaquine phosphate and zmizol had best killing effects on E.wenyonii in vitro.When their concn.in the cultured liquid was 2 000 μg/ml and these two medicines acted with E.wenyonii for 6 h,the infestation ratio of erythrocyte was obviously decreased by below 30%.The oxytetracycline hydrochloride,enrofloxacin,bernier and sulfonamides had some action on the eperythrozoon.The gentamicin sulfate had stronger antibacterial effect,but it showed no killing effect on E.wenyonii.[Conclusion] This study provided the theoretical basis on selecting suitable medicine resisting eperythrozoon in the clinic.