摘要
为了解卵母细胞体外成熟与凋亡过程,进而提高卵母细胞体丰成熟率,本文采自手术标本中卵巢及卵巢组织,吸取4~10mm卵泡,获得未成熟卵母细胞,用正交设计,于培养液中加入不同量组合的人绝经期促性性激素(hMG),卵泡液,颗粒细胞,在37℃,CO25%培养箱中培养,并应用形态学观察和原位DNA片段末端示记(Tunel)检测卵母细胞的凋亡状况,结果:在本研究体外培养条件下能使未成熟卵母细胞成熟,受精。
To study human oocytes maturation and apoptosis in vitro,and increase rate of oocytes matu-ration, immature oocytes obtained from surgically removed ovaries or ovarian tissue were incubat-ed to mature in Ham's FlO with different concentration of hMG, follicular fluid and granulosa cells by an orthogonal design;morphological examination and Tunel method were employed to de-tect apoptosis of oocytes. Results;Immature oocytes can mature and the mature oocytes can be fertilized .and the fertilized ova can develop into early blastocysts in vitro;The medium consisted of 0. 15 lU/nil hMG,40% follicular fluid and 102/ml granulosa cells gave the highest rate of mat-uration, fertilization and cleavage,and the lowest rate of apoptosis (P<0. 05..P<CO. 005). These results suggest that culture medium consisted of hMG .follicular fluid and granulosa cells in suit-abe concentations can promote immature oocytes to become mature in vitro. and the mature oocytes can be fertilized to reach the early blasocyst stage.
出处
《生殖与避孕》
CAS
CSCD
北大核心
1998年第6期346-349,共4页
Reproduction and Contraception